DNA

Part:BBa_K300997:Design

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-02)

AvrII/XbaI mixed site (when XbaI comes from RFC10 Prefix)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA scar designed considering AvrII restriction site and XbaI restriction site in RFC10 BioBrick Prefix.

Source

Datasheets of the commercially available AvrII and XbaI restriction enzymes and the RFC10 BioBrick Prefix sequence.

Very similar to BBa_B0104, but with an additional "G" nucleotide at the 3' end.

References

http://www.roche-applied-science.com/pack-insert/0674257a.pdf

https://parts.igem.org/Help:BioBrick_Prefix_and_Suffix